Anchrom HPTLC

  As we all know, Chromatography is a science of separation of mixtures into its components. There are several types of chromatography, each differing in the kind of stationary phase and mobile phase they use. There are many chromatographic techniques like HPLC, HPTLC and GC. Let us understand what is the resolution in Thin Layer Chromatography? Many times we use the term ‘Resolution’ but what exactly does it mean in TLC/HPTLC?      In Chromatography resolution is ability (of the method) to separate two peaks. In Thin Layer Chromatography or  High-Performance Thin Layer Chromatography , as it is a visual technique, we can also say it is the ability of the method to separate two bands or two peaks. Resolution is termed good when there is a difference of Rf 0.05 between two bands. Ideal resolution is considered when there is base to base separation between adjacent bands. What are the factors affecting resolution in high performance thin layer chromatogr...

  How Do You Test For Colorless Substances In Chromatography? by  Anchrom Lab   Thin Layer Chromatography/High Performance Thin Layer Chromatography is suitable for detection of organic and nonvolatile compounds. There are many compounds which do not have chromophoric group therefore we can not see them with naked eyes. Sometimes these compounds have UV/fluorescence. Such compounds can be detected by using suitable instrument like UV cabinet or CAMAG visualizer which can detect compounds in short UV i.e., 254nm, long UV i.e., 366nm and white light or if any compound is not UV active, a technique of derivatization can be used to make them visible. UV detection, spectra and derivatization are powerful tools which helps in detection of colorless substances in  High Performance Thin Layer Chromatography technique. How colorless substances are analyzed by HPTLC? HPTLC is a stepwise technique. One has to follow certain sequence while doing analysis. First step is applicati...

  Chromatography For Plant Pigments by  Anchrom Lab  |  Sep 1, 2022 How chromatography is used to study plant pigments ? Words ‘Plant pigments’ and ‘chromatography’ reminds us of the beginning of chromatographic science. A Russian Botanist ‘Mikhail Tsvet’ invented chromatography in 19 th  century during his research on separation of plant pigments.  Tsvet had managed to separate a mixture of plant pigments, including chlorophyll, on a column packed with finely ground calcium carbonate, using petroleum ether as the mobile phase. Natural pigments such as chlorophylls, chlorophyll derivatives, xanthophylls, carotenoids, anthocyanins, betacyanin’s are abundant in plants or plant derived products.  Plant parts such as coloured flowers and fruits are because of carotenoids, flavonoids, anthocyanins or betalains. There are two main groups of betalains, the red-violet betacyanins and the yellow betaxanthins. Anthocyanins can be extracted by using solvent like ...

  Why HPTLC Is The Best Choice For Analysis Of Herbal Samples? HPTLC Method for Herbal Medicines Herbal medicines are drugs which are originated from herbal ingredients. As herbal medicines are complex mixtures of many different types of components, it is very difficult and challenging task to analyze them. Quality control, authenticity of the samples/products, safety concerns are difficult questions to answer affecting a huge market and covering many fields of herbal industry like Nutraceutical, Ayurvedic drugs, dietary supplements, Siddha drugs, Homeopathic drugs etc. When herbal samples are in raw material form i.e., crude drug in the form of flowers, fruit, stem, root or leaves it is possible to identify the same by means of macroscopic and microscopic characteristics. However, this type of study does not give much information about its chemical composition other than its authenticity or identity. This composition can be affected by age, environmental factors such as mineral nu...

  Chromatography technique that uses paper sheets or strips as the adsorbent being the stationary phase through which a solution is made to pass is called paper chromatography. It is an inexpensive method of separating dissolved chemical substances by their different migration rates across the sheets of paper. Although paper chromatography technique is simple and rapid, uses minimum amount of material, comparatively less expensive than other chromatographic technique it has many disadvantages. Paper chromatography is useful only for qualitative evaluation. It has limitation to separate complex mixtures. The main differences between TLC and paper chromatography are: The principle behind thin-layer chromatography is based on adsorption. On the other hand, the principle of paper chromatography is based on partition. TLC analysis provides better separation for the components in the mixture and more useful chromatograms than paper chromatography. However, basic TLC has many limitations ...

  What are alkaloids? Alkaloids are a class of basic, naturally occurring organic compounds that contain at least one nitrogen atom.  Alkaloids have  diverse  and important physiological effects on humans and other animals. Well-known alkaloids include  morphine ,  strychnine ,  quinine ,  ephedrine , and  nicotine , piperine, caffeine etc. Many alkaloids have commercial importance as they have different medicinal properties. Alkaloids are found primarily in  plants  and are especially common in certain families of flowering plants.   Alkaloid names generally end in the suffix  -ine , a reference to their chemical classification as amines. In their pure form most alkaloids are colourless, non-volatile, crystalline  solids . Methods for extraction of alkaloids: Alkaloids can be extracted from plant tissues using weak acidic solvents and can then be selectively precipitated from such extracts using ammonia. Alkaloids ca...