Anchrom HPTLC

  How Do You Test For Colorless Substances In Chromatography? by  Anchrom Lab   Thin Layer Chromatography/High Performance Thin Layer Chromatography is suitable for detection of organic and nonvolatile compounds. There are many compounds which do not have chromophoric group therefore we can not see them with naked eyes. Sometimes these compounds have UV/fluorescence. Such compounds can be detected by using suitable instrument like UV cabinet or CAMAG visualizer which can detect compounds in short UV i.e., 254nm, long UV i.e., 366nm and white light or if any compound is not UV active, a technique of derivatization can be used to make them visible. UV detection, spectra and derivatization are powerful tools which helps in detection of colorless substances in  High Performance Thin Layer Chromatography technique. How colorless substances are analyzed by HPTLC? HPTLC is a stepwise technique. One has to follow certain sequence while doing analysis. First step is applicati...

  HPTLC as an analytical technique has applications in multiple fields. It can be used for qualification and quantification of samples in sectors like Pharmaceuticals, Herbals, Cosmetic, Food and Forensic sciences and it can also be used as a fingerprint analysis technique in the above fields.  HPTLC instrumentation  involves different modules for application, development, image documentation and detection. Application of HPTLC in pharmaceutical field: As all the compounds which are analysed by TLC can be analysed using HPTLC, it has a wide variety of applications in the Pharmaceutical field. 1.         Impurity profiling 2.         Content Uniformity 3.         Forced Degradation 4.         Stability 5.         Limit test 6.         Assay Validation...

  High Performance Thin Layer Chromatography (HPTLC) is chromatographic technique based on the principle of planar chromatography. It is a modernized version of Thin Layer Chromatography (TLC) with enhanced capabilities. CAMAG is Swiss based company manufacturing TLC since 1961. They are the world leaders in instrumental HPTLC. First Manual TLC was into existence. Over the years the technique has evolved from manual to semi-automatic which now has upgraded to fully automated HPTLC PRO system in 2019 for routine quality control. HPTLC not only achieves miniaturization of chromatograms but most importantly better resolution and reproducibility with a state of art software for documentation and control over the instrumentation. It is cost effective with high throughput. Up to 15 samples can be run on plate at a time and with less solvent consumption. CAMAG HPTLC  has modules of instruments combined to form a system. Each step has a designated module catering to analyst...

HPTLC High Performance Thin Layer Chromatography is a modernized version of TLC technique. The underlying principle remains the same i.e. planar chromatography. HPTLC gives better separation and resolution of bands compared to TLC technique. Also, quantification aspect is added in HPTLC which an analyst won’t achieve using conventional TLC. HPTLC has a wide area of applications ranging from herbal, food, pharmaceuticals to forensics. Let us have a look at what kind of analyses can be done using  CAMAG HPTLC . Application of herbals involve HPTLC fingerprinting where there is no requirement of marker/standard. Fingerprint is the individual chromatographic track representing all the constituents in a plant sample. This allows us to differentiate at species to species level. Class of compounds is a phytochemical test confirming the presence of functional groups such as phenols, flavonoids, alkaloids and antioxidants etc. This type of analysis is qualitative. Applications in the field ...