Anchrom HPTLC

  What Is HPTLC Fingerprinting ? High-Performance Thin Layer Chromatography (HPTLC) is a modern version of TLC wherein the principle is the same but practice is different. HPTLC is GLP/USP/EP compliant technique . USP’s (United States Pharmacopoeia) definition of HPTLC fingerprint is ‘It is the ( electronic ) image of the visual HPTLC chromatogram. Images are taken at 254nm, 366nm and in white light using suitable TLC photo docsystemstion system like CAMAG visualizer . Fingerprint is evaluated based on Rf, color and intensity of the separated bands. While developing fingerprint our aim should be to separate maximum number of compound with good resolution. For fingerprint development, BRM (Botanical Reference Material) and other related or confounding species are used for comparison. The same species of interest from different geographical or climatic conditions are also used to check variation in the samples. C. rotundus is a traditional herbal medicine i.e. used for the treat...

  Why Is It Challenging To Analyze Food Samples? As food/ food components are very complex in nature, analysis of foods is a very challenging sector. Food samples often come with heavy matrices due to the presence of primary metabolites like carbohydrates, lipids and proteins. Besides this, there may be other complex components which may interfere with the analyte of interest. A number of analytical instruments are used for the analysis of food samples. If we talk about chromatography, instruments like HPTLC, HPLC and GC are most commonly used depending on the aim/type of analysis. HPTLC technique can be used not just for traditional food samples but also for recent categories of food samples like functional foods, nutraceuticals and dietary samples. The Role Of HPTLC In Food Analysis CAMAG HPTLC , a very flexible, cost-effective and fast technique is very useful for the analysis of food samples. Validated HPTLC methods fulfil the requirements for reliable food analysis in many foo...

  THE RETARDATION FACTOR (Rf) – Concept & calculation in TLC/HPTLC: Concept Of Rf Value In TLC/HPTLC: In TLC/HPTLC, basically, two phases are important i.e. stationary phase and mobile phase. The gas phase also plays an important role if we are giving saturation. Nowadays stationary phase is mostly silica gel and mobile phase selection depends on the analyte of interest. Usually, it could be a mixture of binary, tertiary or quaternary solvents. The compound/Analyte of interest is dissolved in a suitable diluent, and applied onto the stationary phase in the form of spots/bonds and the plate is developed in a suitable mobile phase. The analyte and the mobile phase move over the stationary phase due to the capillary action. The differential mobility of different compounds is achieved by the relative affinity of compounds towards the stationary phase and mobile phase. The compounds with a higher affinity towards the stationary phase move slowly as the stationary phase retains the r...

  How to Perform Reverse Engineering in Pharmaceuticals? Reverse engineering/deformulation is a complex process consisting of several phases, such as the identification and quantification of formulation components. It is used at the beginning of the development of a generic drug product.  It is generally performed on an innovator’s formulation. This is performed at the laboratory level for the identification, quantification and characterization of API and excipients used. This can be performed by using various analytical techniques. From the information obtained after doing analysis, one will get a fair idea about how to formulate a product. Reverse engineering is a method that is used to reproduce and redesign an existing product. By using different methods, the design of the product can be studied and the product can be improved. How HPTLC is useful for Reverse Engineering? C amag HPTLC in pharmaceutical analysis  is one of the effective techniques for various phar...

  As we all know, Chromatography is a science of separation of mixtures into its components. There are several types of chromatography, each differing in the kind of stationary phase and mobile phase they use. There are many chromatographic techniques like HPLC, HPTLC and GC. Let us understand what is the resolution in Thin Layer Chromatography? Many times we use the term ‘Resolution’ but what exactly does it mean in TLC/HPTLC?      In Chromatography resolution is ability (of the method) to separate two peaks. In Thin Layer Chromatography or  High-Performance Thin Layer Chromatography , as it is a visual technique, we can also say it is the ability of the method to separate two bands or two peaks. Resolution is termed good when there is a difference of Rf 0.05 between two bands. Ideal resolution is considered when there is base to base separation between adjacent bands. What are the factors affecting resolution in high performance thin layer chromatogr...

  How Do You Test For Colorless Substances In Chromatography? by  Anchrom Lab   Thin Layer Chromatography/High Performance Thin Layer Chromatography is suitable for detection of organic and nonvolatile compounds. There are many compounds which do not have chromophoric group therefore we can not see them with naked eyes. Sometimes these compounds have UV/fluorescence. Such compounds can be detected by using suitable instrument like UV cabinet or CAMAG visualizer which can detect compounds in short UV i.e., 254nm, long UV i.e., 366nm and white light or if any compound is not UV active, a technique of derivatization can be used to make them visible. UV detection, spectra and derivatization are powerful tools which helps in detection of colorless substances in  High Performance Thin Layer Chromatography technique. How colorless substances are analyzed by HPTLC? HPTLC is a stepwise technique. One has to follow certain sequence while doing analysis. First step is applicati...