Anchrom HPTLC

    Thin Layer Chromatography TLC Principle Procedure     What is Chromatography? Chromatography means the separation of mixture of compounds. The components travel through a stationary phase at different speeds and get separated. The mixture is dissolved in the mobile phase and is carried through the stationary phase. Thin Layer chromatography is a form of planar chromatography. The stationary phase is a TLC plate (silica gel) or alumina. The plate is run through a solvent which is known as the mobile phase. The principle of TLC is  where mixture components are separated between a fixed stationary phase and a liquid mobile phase by differential affinities between the two phases. The separation is through adsorption. Thin-layer chromatography can be used to monitor the progress of a reaction, identify compounds present in a given mixture, and determine the purity of a substance.   What is High-Performance Thin Layer Chromatography? HPTLC (Hi...

  Chromatography is used to separate mixtures. This separation technique is important to life science industry. It is used to identify and quantify the metabolites such as pigments, proteins, blood samples, viscera samples. Varied chromatographic techniques are available. HPTLC  is a versatile technique which can be used in wide area of applications. Low-cost HPTLC is the major reason why chromatographers are switching to HPTLC.   Let us learn about the  HPTLC chromatography application  in biological studies. Biological studies include analysing compounds such as amino acids, lipids, carbohydrates, bile acids, vitamins, in blood samples, urine samples and viscera samples.   Sample preparation is the main step. Removing interferences is important and crucial step of any chromatographic analysis. HPTLC proves to be right choice for such analysis. We can change as many HPTLC layers (stationary phase) as we want as they are disposable. HPTLC can be used to ide...

Thin Layer Chromatography (TLC)  is a separation technique used to separate compounds in a mixture. It is based on the principle  of separation through adsorption type. Let us learn about the advantages of Thin Layer Chromatography and compare them with other chromatographic techniques. It is a  cost-effective technique, fast and simple  to use the technique. According to USP 203 Chapter, we can apply 15 samples on one 20x10 cm HPTLC plate. This allows us to simultaneously analyze 15 samples in one go. HPTLC  vs Liquid Chromatography  - Being an open technique, it is robust and does not damage the system as the plates are disposable this gives you an advantage over HPLC. In HPLC, the columns can have a carry-over effect if not cleaned properly. HPTLC is hassle-free and fast. HPTLC vs Gas Chromatography  - Gas chromatography is used to analyze volatile compounds whereas HPTLC is used for non-volatile compounds. HPTLC vs Paper Chromatography  - HPTL...

Nutraceuticals a recent trend in the current health market. They are naturally derived products which are extracted from their original food matrix. They range from isolates, novel foods, products of botanical origin and many. Examples of nutraceutical products are dietary supplements, natural extracts, multi vitamins, fortified dairy products, botanicals and many others. FSSAI has authorized 400 plant extracts to be used as nutraceuticals. How HPTLC can be useful for Nutraceutical Analysis? Identification The first chemical test for any natural or synthetic products is its identification. Botanical origin nutraceuticals, being complex extracts pose a major problem here. However, in USA & Europe “High Performance Thin Layer Chromatography (HPTLC) fingerprint” is the regulatory technique for the purpose. Nutraceuticals are considered as foods by the FSS Act, 2006. Since they are of botanical origin. HPTLC is the only chromatographic technique suitable for routine quality control by ...

  TLC/HPTLC Photodocumentation High Performance Thin Layer Chromatography (HPTLC) is a visual chromatographic technique. What do we mean by the visual approach? The chromatograms, i.e., HPTLC plates, are observed after development. The human eye can see visible spots/bands. If the bands are UV active, we need to observe them under UV. Hence, the HPTLC plate can be visually evaluated, which sets it apart from other chromatographic techniques. The results are monitored and documented. How are the results observed/ documented? HPTLC/TLC results are visual and can be observed by the HPTLC Photodocumentation system. For the HPTLC photodoc, CAMAG has modules such as CAMAG TLC visualizer 2. It has a digital CCD camera. The TLC visualizer electronically captures the high-resolution images of chromatograms under white light, UV 254nm, and under fluorescence. It merges three images to give the best image possible, aided by software-controlled visionCATS for documentation. The software has im...

  High Performance Thin Layer Chromatography (HPTLC) is a visual chromatographic technique. What do we mean by the visual approach? The chromatograms, i.e., HPTLC plates, are observed after development. The human eye can see visible spots/bands. If the bands are UV active, we need to observe them under UV. Hence, the HPTLC plate can be visually evaluated, which sets it apart from other chromatographic techniques. The results are monitored and documented. How are the results observed/ documented? HPTLC/TLC results are visual and can be observed by the HPTLC Photodocumentation system. For the HPTLC photodoc, CAMAG has modules such as CAMAG TLC visualizer 2. It has a digital CCD camera. The TLC visualizer electronically captures the high-resolution images of chromatograms under white light, UV 254nm, and under fluorescence. It merges three images to give the best image possible, aided by software-controlled visionCATS for documentation. The software has image enhancement tools. The da...

 Herbal drugs are gaining popularity nowadays as it has a reputation of being clean, organic and safe for consumption due to their natural source and ingredients. Herbal drugs are used as supplements. They consist of health systems such as Ayurveda, Unani, Siddha, Homeopathy and Naturopathy. Herbal medicines/drugs are plant-derived materials with therapeutic and different health benefits for humans, which are acquired or captured from the plants and their part. Any drug before being brought into the market has to be tested and clinical trials have to be taken. Herbal drugs are more prone to adulteration. Many times, it is a polyherbal formulation meaning it is made up of different herbs/ plants. HPTLC is an efficient tool for herbal drug analysis. The herbal matrix is complex in nature and since HPTLC has disposable layers there is no damage to the instrument. Hence, HPTLC is used in herbal drug standardisation. These medicines are prone to adulteration by notorious dealers and...