What is the purpose of chromatography in biological research?

 



Chromatography is used to separate mixtures. This separation technique is important to life science industry. It is used to identify and quantify the metabolites such as pigments, proteins, blood samples, viscera samples. Varied chromatographic techniques are available.

HPTLC is a versatile technique which can be used in wide area of applications. Low-cost HPTLC is the major reason why chromatographers are switching to HPTLC.

 

Let us learn about the HPTLC chromatography application in biological studies.

Biological studies include analysing compounds such as amino acids, lipids, carbohydrates, bile acids, vitamins, in blood samples, urine samples and viscera samples.  

Sample preparation is the main step. Removing interferences is important and crucial step of any chromatographic analysis. HPTLC proves to be right choice for such analysis. We can change as many HPTLC layers (stationary phase) as we want as they are disposable.

HPTLC can be used to identify and quantify amino acids samples in various matrices. For example, amino acids were isolated and identified from fresh coconut water and preserved market samples.

HPTLC has applications in vitamins analysis from animal and plant sources. For example, vitamin- D3  identification and quantification from fish oils.

Human viscera and blood samples can be analysed by HPTLC for detection of pesticides or any other drugs.

 

Anchrom Enterprises has developed and worked with many Scientists from all over India for HPTLC applications in Forensic Science.

It is India-specific HPTLC Applications Research Centre based in Mumbai. Please contact us at lab@anchrom.in. 

Comments

Post a Comment

Popular posts from this blog

How do You Test for Colorless Substances in Chromatography?

Image
  How Do You Test For Colorless Substances In Chromatography? by  Anchrom Lab   Thin Layer Chromatography/High Performance Thin Layer Chromatography is suitable for detection of organic and nonvolatile compounds. There are many compounds which do not have chromophoric group therefore we can not see them with naked eyes. Sometimes these compounds have UV/fluorescence. Such compounds can be detected by using suitable instrument like UV cabinet or CAMAG visualizer which can detect compounds in short UV i.e., 254nm, long UV i.e., 366nm and white light or if any compound is not UV active, a technique of derivatization can be used to make them visible. UV detection, spectra and derivatization are powerful tools which helps in detection of colorless substances in  High Performance Thin Layer Chromatography technique. How colorless substances are analyzed by HPTLC? HPTLC is a stepwise technique. One has to follow certain sequence while doing analysis. First step is application of sample on sui
Read more

Factors Affecting Resolution in Chromatography

Image
  As we all know, Chromatography is a science of separation of mixtures into its components. There are several types of chromatography, each differing in the kind of stationary phase and mobile phase they use. There are many chromatographic techniques like HPLC, HPTLC and GC. Let us understand what is the resolution in Thin Layer Chromatography? Many times we use the term ‘Resolution’ but what exactly does it mean in TLC/HPTLC?      In Chromatography resolution is ability (of the method) to separate two peaks. In Thin Layer Chromatography or  High-Performance Thin Layer Chromatography , as it is a visual technique, we can also say it is the ability of the method to separate two bands or two peaks. Resolution is termed good when there is a difference of Rf 0.05 between two bands. Ideal resolution is considered when there is base to base separation between adjacent bands. What are the factors affecting resolution in high performance thin layer chromatography? HPTLC is modern version of T
Read more

Automate Your Analytical Processes with the Continuous Flow Analyzer

Image
  Automatic Continuous Flow Wet Chemistry Analyzer In the world of analytical chemistry, efficiency and reliability are paramount. Laboratories dealing with various sample matrices, such as sea water, drinking water, wastewater, environmental samples, soil, food, beverages, tobacco, and more, face the challenge of testing different parameters accurately and efficiently. Manual sample preparation methods can be complex and time-consuming. However, a revolutionary solution has emerged— the AMS Alliance FUTURA 3 Continuous Flow Analyzer. This cutting-edge instrument automates the testing process, providing a complete and reliable solution for laboratories seeking streamlined operations. Each FUTURA 3 console integrates a high precision 3-speed peristaltic pump (medium, fast (startup and washout more speed) and slow (for low consumption of reagents)), a removable drawer for reagents, an analytical manifold, a heater, a detector, and a built-in display for the presentation of essential diag
Read more