HPTLC Fingerprinting

 High Performance Thin Layer chromatography is a versatile technique with applications in herbals, food, pharmaceuticals, nutraceuticals.

 

What is HPTLC Fingerprinting?

HPTLC Fingerprinting is the representation of the phytochemical composition of a plant extract or formulation, in the form of an image at 254nm, 366nm and white light. It is a sequence of peaks or zones of a chromatogram specific for a sample.

United States Pharmacopoeia definition of HPTLC Fingerprint is “It is the electronic image of the visual HPTLC chromatogram.” HPTLC fingerprint is evaluated based on Rf, colour and relative intensity of bands in the electronic image.

For comparison BRM (Botanical Reference Material) is used. If BRM is not available a sample can be collected from the field/market sample is sent to recognized botanical institute e.g., NBRI Lucknow, Agharkar Research Institute Pune, Botanical survey of India western circle Pune. etc. for authentication and that can be used as BRM.

Phytochemical reference standards (RS) are also applied along with samples. Respective plant samples/ raw materials collected from different geographical locations/ climatic conditions can be applied to check the variation in species.

While developing HPTLC fingerprint for certain plant material aim should be to develop fingerprint which shows maximum no. of phytoconstituents with good resolution.

 

HPTLC for Herbal Chromatographic Fingerprinting

HPTLC technique can be used for herbal fingerprinting. Each plant has a different fingerprint pattern. When the plant extract is run in a suitable mobile phase the separation pattern observed is the herbal fingerprint of the said plant. HPTLC is a preliminary step to identify phytochemical compounds and secondary metabolites of a plant.

Fingerprints play an important role in quality control of herbal medicine. It can be used for authentication of raw material or detection of confounded material/ substitutes.

Herbal products and drugs are gaining momentum and so is the substandard material being added to gain profits by manufacturers. Consumers are growing towards organic products. HPTLC plays a major role in detection of such adulterated material.

Herbal Fingerprinting is the technique used for the qualitative and quantitative analysis of herbal components in drugs. Chromatographic fingerprinting is used for both establishing the identity and quality of the herbs being added into formulation.

With a sample size of more than 100 samples in parallel, using Chromatographic Fingerprinting is not only fast and effective, but also economically flexible. Modern chromatographic fingerprinting is used at dual stages during the manufacture of an herbal formula. An initial fingerprinting is carried out for each of the herbs being added to the formulation.

Once the identity and purity of the herb is established all the herbs are incorporated in suitably processed to produce the final poly herbal formulation. A chromatographic fingerprint of the poly herbal formulation is developed after the development of the poly herbal formulation.

 

An HPTLC analysis is useful to measure the purity, strength and consistency of the chemical profile. HPTLC adoption as compared to Gas Chromatography and High-Performance Liquid Chromatography, produces faster results in the standardisation of a consistent biological activity.

If you want to perform HPTLC and Herbal fingerprinting for your business, we provide CAMAG HPTLC machines at an affordable price. Contact us for a quote today. 

Comments

Post a Comment

Popular posts from this blog

How do You Test for Colorless Substances in Chromatography?

Image
  How Do You Test For Colorless Substances In Chromatography? by  Anchrom Lab   Thin Layer Chromatography/High Performance Thin Layer Chromatography is suitable for detection of organic and nonvolatile compounds. There are many compounds which do not have chromophoric group therefore we can not see them with naked eyes. Sometimes these compounds have UV/fluorescence. Such compounds can be detected by using suitable instrument like UV cabinet or CAMAG visualizer which can detect compounds in short UV i.e., 254nm, long UV i.e., 366nm and white light or if any compound is not UV active, a technique of derivatization can be used to make them visible. UV detection, spectra and derivatization are powerful tools which helps in detection of colorless substances in  High Performance Thin Layer Chromatography technique. How colorless substances are analyzed by HPTLC? HPTLC is a stepwise technique. One has to follow certain sequence while doing analysis. First step is application of sample on sui
Read more

Factors Affecting Resolution in Chromatography

Image
  As we all know, Chromatography is a science of separation of mixtures into its components. There are several types of chromatography, each differing in the kind of stationary phase and mobile phase they use. There are many chromatographic techniques like HPLC, HPTLC and GC. Let us understand what is the resolution in Thin Layer Chromatography? Many times we use the term ‘Resolution’ but what exactly does it mean in TLC/HPTLC?      In Chromatography resolution is ability (of the method) to separate two peaks. In Thin Layer Chromatography or  High-Performance Thin Layer Chromatography , as it is a visual technique, we can also say it is the ability of the method to separate two bands or two peaks. Resolution is termed good when there is a difference of Rf 0.05 between two bands. Ideal resolution is considered when there is base to base separation between adjacent bands. What are the factors affecting resolution in high performance thin layer chromatography? HPTLC is modern version of T
Read more

Automate Your Analytical Processes with the Continuous Flow Analyzer

Image
  Automatic Continuous Flow Wet Chemistry Analyzer In the world of analytical chemistry, efficiency and reliability are paramount. Laboratories dealing with various sample matrices, such as sea water, drinking water, wastewater, environmental samples, soil, food, beverages, tobacco, and more, face the challenge of testing different parameters accurately and efficiently. Manual sample preparation methods can be complex and time-consuming. However, a revolutionary solution has emerged— the AMS Alliance FUTURA 3 Continuous Flow Analyzer. This cutting-edge instrument automates the testing process, providing a complete and reliable solution for laboratories seeking streamlined operations. Each FUTURA 3 console integrates a high precision 3-speed peristaltic pump (medium, fast (startup and washout more speed) and slow (for low consumption of reagents)), a removable drawer for reagents, an analytical manifold, a heater, a detector, and a built-in display for the presentation of essential diag
Read more