What Is The Rf Value In HPTLC?
THE RETARDATION FACTOR (Rf) – Concept & calculation in TLC/HPTLC: Concept Of Rf Value In TLC/HPTLC: In TLC/HPTLC, basically, two phases are important i.e. stationary phase and mobile phase. The gas phase also plays an important role if we are giving saturation. Nowadays stationary phase is mostly silica gel and mobile phase selection depends on the analyte of interest. Usually, it could be a mixture of binary, tertiary or quaternary solvents. The compound/Analyte of interest is dissolved in a suitable diluent, and applied onto the stationary phase in the form of spots/bonds and the plate is developed in a suitable mobile phase. The analyte and the mobile phase move over the stationary phase due to the capillary action. The differential mobility of different compounds is achieved by the relative affinity of compounds towards the stationary phase and mobile phase. The compounds with a higher affinity towards the stationary phase move slowly as the stationary phase retains the respe