Factors Affecting Resolution in Chromatography
As we all know, Chromatography is a science of separation of mixtures into its components. There are several types of chromatography, each differing in the kind of stationary phase and mobile phase they use. There are many chromatographic techniques like HPLC, HPTLC and GC. Let us understand what is the resolution in Thin Layer Chromatography? Many times we use the term ‘Resolution’ but what exactly does it mean in TLC/HPTLC? In Chromatography resolution is ability (of the method) to separate two peaks. In Thin Layer Chromatography or High-Performance Thin Layer Chromatography , as it is a visual technique, we can also say it is the ability of the method to separate two bands or two peaks. Resolution is termed good when there is a difference of Rf 0.05 between two bands. Ideal resolution is considered when there is base to base separation between adjacent bands. What are the factors affecting resolution in high performance thin layer chromatography? HPTLC is modern version of T