Why Would One Use TLC Over Paper Chromatography?
Chromatography technique that uses paper sheets or strips as the adsorbent being the stationary phase through which a solution is made to pass is called paper chromatography. It is an inexpensive method of separating dissolved chemical substances by their different migration rates across the sheets of paper. Although paper chromatography technique is simple and rapid, uses minimum amount of material, comparatively less expensive than other chromatographic technique it has many disadvantages. Paper chromatography is useful only for qualitative evaluation. It has limitation to separate complex mixtures. The main differences between TLC and paper chromatography are: The principle behind thin-layer chromatography is based on adsorption. On the other hand, the principle of paper chromatography is based on partition. TLC analysis provides better separation for the components in the mixture and more useful chromatograms than paper chromatography. However, basic TLC has many limitations in m